平菇子实体分化发育相关基因片段的克隆
Cloning of Differentiation and Development Related Gene Fragm ents in Pleurotus ostreatus
马爱民 关海山
摘 要：采用RAP-PCR差异显示技术对平菇菌丝体及原基、菇蕾和成熟子实体进行研究,获得了在原基阶段差异表达的3条cDNA片段POD1、POD2与POD3,经过回收、重扩增、克隆与鉴定后进行测序分析,初步的结果表明3条差异片段的长度分别为745bp、482bp与477bp.同时,国际联网检索查明片段POD3为HSP70基因片段.
关键词：平菇; 差异显示; RAP?PCR; 分化发育相关基因; HSP70
分类号：Q344+.3; S646.1+4 文献标识码：A
基金项目：国家自然科学基金资助项目(39770530)
作者简介：马爱民,男,1965年生,硕士,副教授.
作者单位：马爱民（华中农业大学植物保护系武汉 430070）
关海山（香港中文大学生物系香港）
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华中农业大学学报
JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY
2001 Vol.20 No.4 P.299-302
