微生物功能基因组学研究
Microbial Functional Genomics
黄留玉 王恒?拧? 史兆兴 朱厚础 苏国富
关键词：微生物功能基因组学, 药物靶位, 疫苗, 生物功能图谱
分类号：Q933 文献标识码：A
文章编号：0001-6209(2003)03-0432-07
自从1995年流感嗜血杆菌的基因组序列测定完成之后[1],目前已有75种(株)微生物的基因组完成测序,160多种(株)微生物的基因组测序正在进行中[2].随着各种微生物基因组测序工作的不断完成和序列信息的积累,微生物基因组学研究的重点已由结构基因组学向功能基因组学转移.微生物功能基因组学研究不仅要阐明微生物基因组内每个基因的作用或功能,还要研究基因的调节及表达谱,进而从整个基因组及其全套蛋白质产物的结构、功能、机理的高度去了解微生物生命活动的全貌,揭示微生物世界的各种前所未知的规律,并使之为人类和社会服务.……
基金项目：国家重大基础研究发展规划(973)项目(G1999054103)和首都二四八重大创新工程项目(H010210360119)
作者简介：黄留玉(1962-),男,河南省平舆县人,研究员,硕士,主要从事基因工程及微生物学研究.Tel/Fax:86-10-83821044;E-mail:huangly@nic.bmi.ac.cn
作者单位：黄留玉（军事医学科学院生物工程研究所,北京,100071）
王恒?牛?军事医学科学院生物工程研究所,北京,100071）
史兆兴（军事医学科学院生物工程研究所,北京,100071）
朱厚础（军事医学科学院生物工程研究所,北京,100071）
苏国富（军事医学科学院生物工程研究所,北京,100071）
参考文献：
［1］Fleischmann R D, Adams M D, White O, et al. Whole-genome random sequencing and assembly of Haemophilus influenzae RD. Science,1995,269(5223):496～512.
［2］http://www. Tigr.org/tdb/mdb/mdbcomplete.html
［3］Mori H, Isono K, Horiuchi T, et al. Functional genomics of Escherichia coli in Japan. Res Microbial, 2000,151:121～128.
［4］Ogasawara N. Systematic function analysis of Bacillus subtilis genes. Res Microbial, 2000,151:129～134.
［5］Chalker A F, Lunsford R D, Rational identification of new antibacterial drug targets that are essential for viability using a genomics-based approach. Pharmacology & therapeutics, 2002,95:1～20.
［6］Richmond C S, Glasner J D, Mau R, et al. Genome-wide expression profiling in Eschericha coli K-12. Nucleic Acids Res, 1999,27:3821～3835.
［7］Ye R W, Tao W, Bedzyk L, et al. Global gene expression profiles of Bacillus subtilis grown under anaerobic conditions. J Bacterial, 2000,182:4458～4465.
［8］Perrot F, Hebraud M, Junt G A, et al. Protein synthesis in Escherichia coli at 4℃. Electrophoresis, 2000, 21:1625～1629.
［9］Bianchi M M, Ngo S, Vandenbol M, et al. Large-scale phenotypic analysis reveals identical contributions to cell functions of known and unknown yeast genes. Yeast, 2001,18(15):1397～1412.
［10］Ross M P, Coelho P S, Roemer T, et al. Large-scale analysis of yeast genome by transposon tagging and gene disruption. Nature, 1999,402:413～418.
［11］Reinscheid D J, Gottschalk B, Schubert A, et al. Identification and molecular analysis of pcsB, a protein required for cell wall separation of Group B streptococcus. J Bacteriol, 2001,183:1175～1183.
［12］Read T D, Gill S R, Tettelin H, et al. Finding drug targets in microbial. DDT, 2001, 6(17):887～892.
［13］Loferer H. Mining bacterial genomes for antimicrobial targets. Molecular Medicine Today, 2000,6:470～474.
［14］Forsyth R A, Haselbeck R J, Ohlsen K L, et al. A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 2002,43(6):1387～1400.
［15］Foulkes J G. Elitra pharmaceuticals: new paradigms for antimicrobial drug discovery. Drug Discovery Today, 2002,7(5):s12～s15.
［16］Grandi G. Antibacterial vaccine design using genomics and proteomics. TRENDS in Biotechnology, 2001,19(5):181～188.
［17］Tettelin H, Saunders N J, Heldelberg J, et al. Complete genome sequencing of Neisseria Meningitidis serogrous B strain MC58. Science, 2000,287:1809～1581.
［18］Rappppuoli R. Reverse vaccinology. Curr Opin Microbiol,2000,3:445～450.
［19］Chakabari D N, Fiske M J, Fletcher L D, et al. Application of genomics and proteomics for identification of bac-terial gene products as potential vaccine candidates. Vaccine, 2001,19:601～612.
［20］Wassenaar T M, Gaastrs W. Bacterial virulence: can we draw the line? FEMS Microbiology Letters, 2001,201:1～7.
［21］Hayash T, Makino K, Ohnishi M, et al. Complete genome sequence of enterohemorrhagic Eschelichia coli O157:H7 and genomic comparison with laboratory strain K-12. DNA Research, 2001,8:11～22.
［22］Cornelis G R, Rappuoli R. Host-microbe interactions: bacteria microbiology will never be the same again... Current Opinion in Microbiology, 2001,4:13～15.
［23］Francis M S, Wolf W H, forsberg A. Regulation of type Ⅲ secretion systems.Current Opinion in Microbiology, 2002,5:166～172.
［24］Orth K. Function of the yersinia effector YopJ. Current Opinion in Microbiology, 2002,5:38～43.
［25］Cohen P, Bouaboula M, Bellis M, et al. Monitoring cellular responses to Listeria monocytogenes with oligonucleotide arrays.J Biol Chem, 2000, 275:11181～11190.
［26］Ideker T, Thorsson V, Ranish J A, et al. Integrated genomic and proteomic analyses of a systematically perturbed metabolic network. Science, 2001,292(5):929～934.
［27］Bono H, Ogata H, Goto S, et al. Reconstruction of amino acid biosynthesis pathways from the complete genome sequence. Genome Res,1998,277:1453～1474.
［28］Overt M W, Schilling C H, Famili I, et al. Metabolic modeling of microbial. Trend in Biochemical Sciences, 2001,26(3):179～186.
［29］VanBogelen R A, Greis K D, Blumenthal R M, et al. Mapping regulatory networks in microbial cells. Trends in Microbio-logy, 1999,7(8):320～328.
［30］Rain J C, Selig L, Reuse H D, et al. The protein-protein interaction map of Helicobacter pylori. Nature, 2001,409:211～215.??
微生物学报
ACTA MICROBIOLOGICA SINICA
2003 Vol.43 No.3 P.432-438
