裂褶菌产纤维二糖脱氢酶条件优化及
部分酶学性质研究
方靖 刘稳 高培基
摘 要：研究了裂褶菌Schizophyllum communeAS 5.391产纤维二糖脱氢酶的条件。该酶是诱导酶，棉花是最好的诱导底物。培养基中加入琥珀酸钠缓冲液和土温80利于酶的合成。而木素相关物质藜芦醇或愈创木酚对酶的生成没有影响。该酶在pH3.5～10.5和45℃以下保持稳定，其最适作用温度为30℃，最适pH为4.5。Zn2＋和Ag＋对该酶活性有很大的抑制作用，而叠氮化钠和氰化钾对酶活力没有影响。
关键词： 裂褶菌，纤维二糖脱氢酶，酶的合成，酶性质研究
中图分类号： Q939.97 文献标识码： A
文章编号： 1007-3515（2000）01-0107-0110
OPTIMIZATION OF CELLOBIOSE DEHYDROGENASE
PRODUCTION BY SCHIZOPHYLLUM COMMUNE
AND SOME PROPERTIES OF THE ENZYME
FANG Jing, LIU Wen, GAO PeiJi
(The state Key Laboratory of Microbial Technology, Shandong University, Jinan 250100)
ABSTRACT: Effects of various parameters on cellobiose dehydrogenase (CDH) production by Schizophyllum commune AS 5.391 were determined. CDH was induced by cellulosic substrates, and cotton cellulose with high crystallinity supported the maximum CDH production. Addition of succinate buffer and Tween 80 facilitated CDH secretion. The optimized temperature and pH for CDH are 30℃ and 4.5. It keeps stable at pH range of 3.5～10.5, and below 45℃. CDH was inhibited by Zn2＋ and Ag＋, but it was not influenced by azide and cyanide.
KEY WORDS: Schizophyllum commune, Cellobiose Dehydrogenase, Enzyme Production, Characterization
国家自然科学基金（39430020）及山东大学微生物技术国家重点实验室资助项目
方靖（山东大学微生物技术国家重点实验室 济南 250100）
刘稳（山东大学微生物技术国家重点实验室 济南 250100）
高培基（山东大学微生物技术国家重点实验室 济南 250100）
［REFERENCES］
1，Ander P, 1994. The cellobiose-oxidizing enzymes CBQ and CBO as related to lignin and cellulose degradation-a review. FEMS Microbiol Review, 13: 297～312
2，Archibald FS, Bourbonnais R, Jurasek L, et al., 1997. Kraft pulp bleaching and deligninfication by Trametes versicolor. J Biotechnol, 53:215～236.
3，Eriksson KEL, Habu N, Samejima M, 1993. Recent advances in fungal cellobiose oxidoreductases. Enzyme and Microb Technol, 15: 1002～1008.
4，Fang J, Qu YB, Gao PJ, 1997. Wide distribution of cellobiose-oxidizing enzymes in wood-rot fungus indicates a physiological importance in lignocellulosics degradation. Biotechnol Tech, 11: 195～197
5，Kremer SM, Wood PM, 1992a. Continuous monitoring of cellulose oxidation by cellobiose oxidase from Phanerochaete chrysosporium. FEMS Lett, 92: 187～192
6，Kremer SM, Wood PM, 1992b. Production of Fenton's reagent by cellobiose oxidase from cellulolytic cultures of Phanerochaete chrysosporium. Eur J Biochem, 208: 807～814.
