虫草多型现象的5.8S rDNA 和ITS2 序列测定研究
康冀川 梁宗琦 刘爱英 理查Y.C.孔
摘 要：新定名的甘肃虫草Cordycepsgansuensi与冬虫夏草Cordycepssinensi形态相似。蛹虫草Cordycepsmilitaris具有拟青霉型（Paecilomyces-type）和轮枝孢型（Verticillium-type）两类无性型。此研究对5.8SrRNA基因及ITS2间区DNA序列的分析结果表明，甘肃虫草和冬虫夏草的DNA序列相同，属于同一种；蛹虫草的两类无性型具有分子水平上的遗传趋异性。
关键词：冬虫夏草，甘肃虫草，蛹虫草，分子研究，多型现象
分类号：Q939.96 文献标识码：A
文章编号：1007-3515（2000）04-0492-0497
MOLECULAR EVIDENCE OF POLYMORPHISM IN CORDYCEPS BASED ON 5.8S rDNA AND ITS2 SEQUENCES
KANG Ji-Chuan（Department of Plant Pathology, University of Stellenbosch, P.Bag X1, Matieland 7602, South Africa）
LIANG Zong-Qi（Laboratory of Fungus Resources, Guizhou University, Guiyang 550025）
LIU Ai-Ying（Laboratory of Fungus Resources, Guizhou University, Guiyang 550025）
LIU Ai-Ying（Department of Biology and Chemistry, The City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong）
Abstract：Cordyceps gansuensis, C. sinensis and C. militaris are reexamined in this study.Cordyceps gansuensis morphologically resembles C.sinensis, while C.militaris has two different types of anamorphs, the Paecilomyces-type and the Verticillium-type. Analysis of DNA sequences of the 5.8S rRNA gene and partial internal transcribed spacer (ITS2) region of the fungi indicate that C.gansuensis and C.sinensis are conspecific and that the two types of anamorphs of C.militaris are diverse at molecular level.
Keywords：Cordyceps sinensis, C.gansuensis, C.militaris, Molecular studies, Polymorphism.
基金项目：国家自然科学基金资助
作者单位：康冀川（Stellenbosch 大学植物病理系，南非 Matieland 7602）
梁宗琦（贵州大学真菌资源研究室，贵阳 550025）
刘爱英（贵州大学真菌资源研究室，贵阳 550025）
理查Y.C.孔（香港城市大学生物化学系，香港）
参考文献：
［1］Camichael JW, 1981. Biology of conidial fungi. vol 1,Academic press inc.1~514
［2］Devereux J, Haeberli P, Smithies O, 1984. Comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12, 387～395
［3］Felsenstein J, 1993. PHYLIP: phylogeny inference package (version 3.5c). Distributed by the author, Department of Genetics, University of Washington, Seattle, USA
［4］Kobayasi Y, Shimizu D, 1993. Iconography of vegetable wasps nad plant worms. Hoikusha Pub Co Ltd Japan.1~625
［5］Lee SB,Taylor JW, 1990. Isolation of DNA from fungal mycelia and single spores. In PCR Protocols: A Guide to Methods and Applications, ed MA Innis, DH Gelfand, JS Sninsky, TJ White. New York: Academic press. 1～282
［6］Liang ZQ,1990. Anamorph of Cordyceps militaris and artificial culture of its fruitibody. Southwest China Journal of Agricultural Sciences 3, 1～6
［7］Liang ZQ, Liu AY, Liu JL, 1991. A new species of the genus Cordyceps and its Metarhizium anamorph. Acta Mycologica Sinica （真菌学报）10: 172～180
［8］Liang ZQ, Liu AY, Liu JL, 1995. The development of ascospore in Cordyceps sinensis (Berk.) Sacc. Acta
［9］Mycologica Sinica （真菌学报）14: 148～152
［10］Liang ZQ, Liu AY, 1996. The genus Cordyceps and its allies from Kuankuoshui Preserve in Guiahou I. Acta Mycologica Sinica （真菌学报）15: 264～271
［11］Liang ZQ , Liu AY, 1997. The genus Cordyceps and its allies from Kuankuoshui Preserve in Guiahou II. Mycosystema （菌物系统）16: 61～67
［12］Liu AY, Liang ZQ, 1997. Cordyceps spp. and some other entomopathogenic fungi from the Eemei Mountain Preserve in China I. Mycosystema （菌物系统）16: 13～143
［13］Liu ZY, Liang ZQ, 1996. A new species of Paecilomyces isolated from Cordyceps odonatae. Mycosystema （菌物系统）8: 83～87
［14］Liu ZY,LiangZQ, Liu AY, 1997. A new fungus of the genus Cordyceps growing on white grub. Mycosystema （菌物系统）16: 4～8
［15］Saitou N, Nei M, 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406～425
［16］Thompson JD, Higgins DG,Gibson TJ, 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalites and weight matrix choice. Nucleic Acids Research 22: 4673～4680
［17］White TJ, Bruns TD, Lee S,Taylor JW, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols:A Guide to Methods and Applications, ed. MA Innis, DH Gelfand, JS Sninsky, TJ White, New York: Academic. 1～482
［18］Zhang K, Wang C, Yan M, 1989. A new species of Cordyceps from Gansu, China. Transactions of Mycological Society of Japan 30: 295～299
