大球盖菇原生质体再生及单核化特性的研究
闫培生 李桂舫 蒋家惠 黄健
摘 要：大球盖菇原生质体再生条件及单核化特性结果。原生质体再生速度极快，涂布平板3d后肉眼即可见明显的再生菌落形成,在PGPM再生培养基上再生率为0.97～2.0%，渗稳剂种类对再生率无明显影响，但可影响再生菌落形态,液体预培养1～2d，再生率明显下降；大球盖菇原生质体单核化率高达77.6%，且再生双核体和再生单核体在形成再生菌落时无时间差，其生长速度亦无快慢之分，液体预培养可显著减少单核化率，再生单核体中存在亲本两种交配型，但二者的比率不为1。
关键词：大球盖菇，原生质体，单核化
分类号：Q932 文献标识码：A
文章编号：1007-3515（2001）01-0107-0110
REGENERATION AND MONOKARYOTATION OF PROTOPLASTS OF STROPHARIA RUGOSO-ANNULAT
YAN Pei-Sheng（Institute of Applied Mycology, Department of Plant Protection, Laiyang Agricultural University, Laiyang, Shandong 265200）
LI Gui-Fang（Institute of Applied Mycology, Department of Plant Protection, Laiyang Agricultural University, Laiyang, Shandong 265200）
JIANG Jia-Hui（Institute of Applied Mycology, Department of Plant Protection, Laiyang Agricultural University, Laiyang, Shandong 265200）
HUANG Jian（Department of Biology, Qingdao Educational College, Qingdao 266071）
Abstract：Regeneration and monokaryotation of Stropharia rugoso-annulata protoplasts were investigated. The results showed that the protoplast regeneration was very fast, and the regenerated colonies could be seen by naked eyes after plating 3d. The regeneration rate was 0.97～2.00% on PGPM. The osmotic stabilizers of mannitol, sucrose and magnesium sulphate had no significant effects on regeneration rate, but could affect the morphology of regenerated colonies. Pre-culture of protoplasts in liquid regeneration media for 1～2d at 25℃ could decrease the regeneration and monokaryotation rate. The protoplast monokaryotation rate was 77.7%. The regenerated dikaryons and monokaryons could be formed on the plates simultaneously, and had no difference in mycelium growth rate and colony-forming time. Two kinds of parent mating types could be found among protoplasted monokaryons, but the ratio was not 1:1. It was the first report about the regeneration and monokaryotation characters of Strapharia rugoso-annulata.
Keywords：Stropharia rugoso-annulata, Protoplast, Monokaryotation
基金项目：山东省教委资助课题
作者简介：闫培生,E-mail: psyan@lyac.edu.cn
作者单位：闫培生（莱阳农学院植保系应用真菌研究室 山东 莱阳265200； 2青岛教育学院 青岛 266071）
李桂舫（莱阳农学院植保系应用真菌研究室 山东 莱阳265200；）
蒋家惠（莱阳农学院植保系应用真菌研究室 山东 莱阳265200；）
黄健（青岛教育学院 青岛 266071）
参考文献：
［1］闫培生，1998．中国木耳属菌株的分子鉴定及分子系统发育关系研究（博士学位论文）．武汉：华中农大
［2］张树庭，林芳灿，1997．蕈菌遗传与育种．北京：中国农业出版社．
［3］黄年来，1995．大球盖菇的分类地位和特征特性．食用菌6:11
［4］潘迎捷，1994. 异宗结合食用菌原生质体单核化.上海农业学报9(2):1～5
［5］颜淑婉，苏诗垂，1995.大球盖菇人工栽培研究简报.江苏食用菌16(3):2
