赵风云; 林俊芳; 叶泽波; 王艺红; 杨博; 郭丽琼
华南农业大学食品学院生物工程系; 华南农业大学生物质能研究所
【中文摘要】 草菇V23原生质体再生菌丝经过0℃低温胁迫处理108 h后,筛选出菌丝较耐低温的菌株V23a,获得V23a的孢子分离菌株(Spore Isolation Strains,SIS),经栽培后,筛选生物学效率高的菌株进行组织分离,获得组织分离菌株(Tissue Isolation Strains,TIS),选出其中生物学效率最高的Va2作为草菇高产新菌种,其生物学效率比对照V23高14.27%。选育过程中分析了SIS菌株的气生菌丝密度、厚垣孢子数量和出菇率,比较了SIS和TIS菌株的菌丝生长速度、原基数、生产周期、单菇平均质量和生物学效率等主要性状。结果表明,可出菇的SIS菌株其子实体产量差异较大,但均低于对照菌株V23。TIS菌株的产量比SIS的提高了087.18%。
【英文摘要】 Volvariella volvacea,strain V23a,was selected from regenerated protoplasts of strain V.volvacea V23 by exposure to 0 ℃ for 108 h.Spore Isolated Strains(SIS) were obtained in relatively high yield from V23a fruit bodies using standard methodology,and Tissue Isolated Strains(TIS) were then isolated from fruit bodies of the SIS.A new high-yielding(14.27% higher yield compared with strain V23) V.volvacea strain,Va2,was selected from the TIS.Aerial hyphae and chlamydospore production,and fruiting frequency values,were determined for SIS,and mycelial growth rates,densities of primordia,times elapsing between substrate inoculation and harvesting of the fruit bodies,average fruit body weights and Biological Efficiency values were recorded for both SIS and TIS.Fruit body yields for SIS varied considerably and were invariably lower compared to strain V23,whereas yields for TIS were up to 87.2 % higher than those of the corresponding SIS.
【中文关键词】 草菇; 孢子分离; 组织分离; 生物学效率
【英文关键词】 Volvariella volvacea; spore isolation; tissue isolation; Biological Efficiency
【基金】国家高技术研究发展计划“利用食用菌工程菌株创制食品专用的多功能纤维素酶”(863计划,编号:2006AA10Z301);; 广东省科技计划课题“食用菌产业推进关键技术研究与示范”(编号:2008A02010024)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年04期 【DOI】CNKI:SUN:SYJB.0.2009-04-005