尚晓冬; 宋春艳; 沈学香; 徐珍; 谭琦
农业部应用真菌资源与利用重点开放实验室上海市食用菌工程技术研究中心上海市农业遗传育种重点实验室上海市农业科学院食用菌研究所
【中文摘要】 对从国内外收集到的19株杏鲍菇[Pleurotus eryngii(DC.ex.Fr.)Quel]菌株进行随机扩增多态性DNA(RAPD)分析,结果表明,在未采用外类群参考的情况下,供试菌株间的相似系数在0.448到0.857之间,其中栽培菌株的相似系数较高,以0.72为阈值时,分成两个类群:来自台湾(3)、三明(5)、武汉(15、16、17)、上海(19)和日本(1、12)的8个菌株构成一个类群,而另外2株台湾菌株(2、6)和1株武汉菌株(18)以及一株来源不明的13号菌株构成另外一个类群。栽培菌株与其它菌株相似程度较低,而且在子实体形态上也有较明显的差异。
【英文摘要】 Random amplified polymorphic DNA(RAPD) methodology was used to identify 19 strains of Pleurotus eryngii obtained from sources both within China and overseas.A dendrogram based on cluster analysis of RAPD patterns revealed that similarity coefficients ranged from 0.448 to 0.857 and,at the similarity level of 0.72,cultivated strains separated into two groups.Eight strains,No.3 from Taiwan,No.5 from Sanming,Nos.15,16 and 17 from Wuhan,No.19 from Shanghai,and Nos.1 and 12 from Japan formed one group,while the second group consisted of two strains from Taiwan(Nos.2 and 6),a Wuhan strain(No.18),and a strain of unknown origin(No.13).Significant differences in both the RAPD patterns and fruit body morphologies were observed between the cultivated strains and the remaining six test strains.
【中文关键词】 杏鲍菇; RAPD; 子实体; 形态特征
【英文关键词】 Pleurotus eryngii; RAPD; fruit body; morphological characteristics
【基金】上海市科技兴农重点攻关项目[编号:沪农科攻字(2006)第3-1号]的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-001