宋春艳; 尚晓冬; 谭琦; 刘德云; 郑巧平; 项寿南
中华人民共和国农业部应用真菌资源与利用重点开放实验室上海市食用菌工程技术研究中心上海市农业遗传育种重点实验室上海市农业科学院食用菌研究所; 浙江省丽水市农业科学研究所
【中文摘要】 以香菇菌种939和本所实验菌株中19为亲本,进行原生质体单核体分离和配对杂交。对杂交组合和亲本菌株的菌盖直径、厚度,菌柄长度、直径等性状进行考察。结果表明,10个杂交组合在子实体外观形态上与亲本存在明显的差异,且原生质体单核体杂交后代的农艺性状表现规律与亲本交配型的类型存在一定的关联。
【英文摘要】 Protoplast monokaryons were prepared from Lentinula edodes,strains 939 and Zhong-19,and eight and two protoplast monokaryons respectively from the two parent strains were intercrossed after determining the mating type.Ten hybrid offspring strains were selected based on mycelial growth rate and used in fruiting tests.Comparison of fruit body characteristics of both hybrid and parental strains revealed variations in the diameter and thickness of fruit body pilei,and the length and diameter of fruit body stipes,which were related to the mating type.
【中文关键词】 原生质体单核体; 杂交育种; 交配型基因; 性状变异
【英文关键词】 Protoplast monokaryon; crossbreeding; mating type gene; character variation
【基金】上海市科技兴农重点攻关项目[编号:沪农科攻字(2004)第2-16号]的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2008年03期 【DOI】CNKI:SUN:SYJB.0.2008-03-001