BAO Dapeng; CHEN Mingjie; ZHANG Meiyan(Shanghai Biotechnology and Engineering Research Center for the Edible Fungi; Key Laboratory of AgriculturalGenetics and Breeding of Shanghai; Institute of Edible Fungi; Shanghai Academy of AgriculturalSciences; Shanghai; 201106; China)
【中文摘要】 A 531 bp fragment of a putative mitochondrial intermediate peptidase (mip) gene from Lentinula edodes was amplified by PCR using a pair of degenerate primers designed according to conserved sequences of mip genes reported in other Basidiomycetes. Alignment analysis revealed that this putative partial mip gene from L. edodes exhibited high homology with corresponding segments of mip genes from the other Basidiomycetes. Since the gene encoding MIP appears to be very closely linked with the A type ...更多mating gene of Basidiomycetes, identification of mip may prove a useful tool for locating and cloning the A type mating gene in L. edodes and other edible fungi. 还原
【英文摘要】 A 531 bp fragment of a putative mitochondrial intermediate peptidase (mip) gene from Lentinula edodes was amplified by PCR using a pair of degenerate primers designed according to conserved sequences of mip genes reported in other Basidiomycetes. Alignment analysis revealed that this putative partial mip gene from L. edodes exhibited high homology with corresponding segments of mip genes from the other Basidiomycetes. Since the gene encoding MIP appears to be very closely linked with the A type mating gene of Basidiomycetes, identification of mip may prove a useful tool for locating and cloning the A type mating gene in L. edodes and other edible fungi.
【英文关键词】 Lentinula edodes; Mating type gene; A factor; Mitochondrial intermediate peptidase (mip) gene
【基金】Sponsored by project of Shanghai Agriculture Committee ( No.2003-4-5);; project of China AgriculturalMinistry(No .nyhyzx-07-008)
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2007年04期 【DOI】CNKI:SUN:SYJB.0.2007-04-002