WANG Hong; CHEN MingjieKey Laboratory of Agricultural Genetics and Breeding of Shanghai; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China
【中文摘要】 A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.
【英文摘要】 A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.
【英文关键词】 Volvariella volvacea; Cold induction; Cor3 gene; Quantitative PCR; Plasmid; Standard curve
【基金】Sponsored by the Shanghai Academy of Agricultural Science and Shanghai Science Committee (No .003912076)
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2007年03期 【DOI】CNKI:SUN:SYJB.0.2007-03-006