LIU Yong 1; 2 ; GUO Li-qiong 1; 2 ; LIN Jun-fang 1; 2 * 1 Department of Bioengineering; College of Food Sciences; South China Agriculture University; Guangzhou 510642; China; 2 State Key Biotechnology Laboratory for Tropical Crops; Chinese Academy of Tropical Agricultural Sciences; Haikou 571101; China
【英文摘要】 Twelve PCR amplification products, generated by homology cloning using Ganoderma lucidum genomic DNA as the template, were cloned into the T-easy vector and sequenced. Comprehensive analysis using BLASTn, Proscan: version 1.7, and Signal Scan predicted four of the products, LY17.1, LY19.3, LY26 and LY31, to be putative promoters. Of these, LY17.1 and LY26 possessed all the promoter characteristics including promoter core regions, TATA boxes and transcription starting sites (TSS). The sequence of LY31 showed 98% homology with the gpd promoter sequence of Lentinula edodes.
【英文关键词】 Ganoderma lucidum; Promoter; Cloning
【基金】theFoundationofGuangdongProvince(No.2003C31207)
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2006年01期 【DOI】CNKI:SUN:SYJB.0.2006-01-005