江艳华; 江晓路; 王鹏; 王首燕
中国海洋大学生命科学与技术学部食品工程系; 中国海洋大学生命科学与技术学部食品工程系; 中国海洋大学生命科学与技术学部食品工程系; 中国海洋大学生命科学与技术学部食品工程系 青岛 266003 ; 青岛 266003 ; 青岛 266003 ; 青岛 266003
【中文摘要】 研究了白灵菇液体培养条件,确定最佳培养基配方为10%(w/v)麸皮,1%蔗糖,0.3%蛋白胨,0.1%KH2PO4,0.05%MgSO4;最佳培养条件为pH7.0,温度25℃,接种量10%(v/v),培养基装量100mL/250mL,培养时间7d。栽培试验表明,液体菌种制种时间比固体菌种的少13～18d,头潮菇出菇时间缩短了10～20d。
【英文摘要】 The optimal fermentation medium and the optimal culture conditions for submerge-cultured Pleurotus nebrodensis were studied in this paper. The results showed that the optimal fermentation medium was composed of wheat bran 10 % (w/v) , sucrose 1 % , peptone 0.3%, KH2 PO4 0. 1 % , MgSO4 0. 05 % ; the optimal culture conditions were pH 7. 0, inoculation rate 10% (v/v) , medium capacity 100mL/250mL, culture time 7d. The results of comparative cultivation of Pleurotus nebrodensis showed that the preparing time of submerged spawn was 13-18d shorter than that of the solid spawn and the fruitbody formation time of the first flash was 10-20d earlier than that of the solid spawn.
【中文关键词】 白灵菇; 液体培养; 种子制备; 栽培
【英文关键词】 Pleurotus nebrodensis; Submerged fermentation; Spawn preparation; Cultivation
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2005年01期 【DOI】CNKI:SUN:SYJB.0.2005-01-009