阎培生; 罗信昌; 周启
华中农业大学应用真菌研究所!武汉430070; 华中农业大学应用真菌研究所!武汉430070; 华中农业大学生命科学技术学院!武汉430070
【中文摘要】 通过研究培养时间和培养基种类对毛木耳同工酶凝胶电泳图谱的影响,证实酯酶同工酶在毛木耳中是一种稳定表达的酶类,培养时间和培养基种类对其无明显影响;苹果酸脱氢酶同工酶不受培养基种类的影响,但随培养时间的延长,由5～10d时的三条带减为15～25d时的二条带;多酚氧化酶同工酶和过氧化物酶同工酶受培养丛种类的影响显著,但除培养初期酶带数较少外,在10～25d时酶带数稳定不变。由此可筛选最适培养时间和培养基种类,用于木耳属菌株的分类鉴定。
【英文摘要】 Effects of different culture time and medium on the isozyme gel electrophoretic pattern of Auricu-laria polytricha were investigated in this paper. The results showed that the esterase isozyme was a stable expressing enzyme in A . polytricha, its pattern was not significantly affected by the culture time and medium.The malate de-hydrogenase isozyme was not affected by the medium, but with the elongating of the culture time, the band numbers were decreased from 3 in 5- l0d to 2 in 15-25d. The polyphenol oxidase isozyme and peroxidase isozyme were sig-nificantly affected by different media, but the band numbers were stable in 10-25d except that the band numbers were less in early culture time of 5d. These results indicated that we could choose optimal culture time and medium to classify and identify the strains in Auricularia Bull. ex Mer.
【中文关键词】 毛木耳; 同工酶酶谱; 培养时间; 培养基
【英文关键词】 Auricularia polytricha ; Isozyme pattern; Culture time; Medium
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,1999年01期 【DOI】CNKI:SUN:SYJB.0.1999-01-002