潘迎捷; 郑志仁; 李人圭
上海市农业科学院食用菌研究所农业部食用菌遗传育种重点实验室!上海201106; 华东师范大学生物系!上海200062; 华东师范大学生物系!上海200062
【中文摘要】 本文测定了温度对香菇胞外纤维素酶和半纤维素酶活性的影响。木屑基质培养90天的香菇胞外游纸纤维素酶（C_1酶）、羧甲基纤维素酶（C_X酶）和半纤维素酶（H_C酶）的最适作用温度分别是55℃、55～60℃和50℃。在50～55℃条件下保温1小时后,C_1酶和H_C酶活性大幅度下降,而C_x酶活性下降较为缓慢,具有较高的热稳定性。本文提出相对活力系数（Relative activity coefficient,RAC）作为表示香菇菌丝生长温度与酶最适作用温度之间关系的一个参数。
【英文摘要】 The effect of temperature on extracellular ctllulasc and hemicellulase was detected. After 90 days cultivation on sawdust medium, it was found that the best suitable temperature of extracellular cellulaso, extracellular carboxymcthyl cellulase and extracellular hemicellulase was 55℃), 55-60℃ and 50℃ respectively. After 1h treatment at 50-55℃, cellulase and hemicellulase activity dropped significantly, but carboxymethyl cellulase activity decreased slowly. The results indicated that carboxymethyl cellulase was more thermostable. It is considered that the relative activity coefficient could be used as index to show the relationship between Lentinula edodes mycelium growth temperature and the best suitable temperature of enzyjne activity.
【中文关键词】 香菇; 菌丝生长温度; 纤维素酶; 半纤维素酶; 相对活力系数
【英文关键词】 Lentinula edodes: Mycelium growth temperature; Cellulase; Hemicellulase; Relative activity coefficient
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,1996年02期 【DOI】CNKI:SUN:SYJB.0.1996-02-000