徐春燕; 张晓昱; 马富英; 杨娜
华中科技大学生命科学与技术学院分子生物物理教育部重点实验室
【中文摘要】 以糖化率为指标对比白腐菌I.lacteus C与G.lucidum E、褐腐菌Fomitopsis sp.IMER2与P.cocos F预处理玉米秸秆的效果,结果表明:不同菌株处理效果差异很大,白腐菌预处理效果优于褐腐菌,木质纤维素网状结构中木质素去除比纤维素结晶度降低利于提高糖化率。I.lacteus C预处理优势明显,处理30 d糖化率最高,比对照提高135.7%,达62.0%;G.lucidum E处理后仅20 d样品糖化率提高,达42.1%;Fomitopsis sp.IMER2处理后糖化率稍有提高;P.cocos F处理降低糖化率。进一步研究发现I.lacteus C通过改性玉米秸秆提高糖化率:处理30 d可溶性还原糖含量提高287.5%,转化率提高178.3%。
【英文摘要】 In this paper,corn stover saccharification rate was determined to evaluate the effect of biological pretreatment by two kinds of basidiomycetes.Two white rot fungi(I.lacteus C and G.lucidum E) and two brown rot fungi(Fomitopsis sp.IMER2 and P.cocos F) were grown on corn stover for different incubation duration.The results indicated white rot fungi was better than brown rot fungi on enhancing saccharification rate and the effects differed a lot between strains of different varieties and genus,which suggested that,for lignocellulosic biomass,removing lignin was more efficient than reducing cellulose crystallinity for enhancing saccharification rate.Of the four basidiomycetes,I.lacteus C was the best strain for biological pretreatment.The saccharification rate of corn sotver pretreated by I.lacteus C for 30 d reached the highest of 62.0%,which was 135.7% higher than control sample.G.lucidum E enhanced saccharification rate to 42.1% after incubation for 20 d.For brown rot fungi,Fomi-topsis...更多 sp.IMER2 improved the saccharification rates a little,while P.cocos F significantly reduced it.Further study indicated that strain I.lacteus C had the most improved saccharification rate by significantly enhancing soluble reducing sugars contents and transformation rates of corn stover,which were respectively 287.5% and 178.3% higher than control sample.The high sac-charification rate of pretreated corn stover by I.lacteus C had great potential in biological process. 还原
【中文关键词】 生物预处理; 白腐菌; 褐腐菌; 木质纤维素; 糖化
【英文关键词】 Biological pretreatment; White rot fungi; Brown rot fungi; Lignocellulose; Saccharification
【基金】国家973项目(项目编号2007CB210200);; 863计划(项目编号2007AA021301)
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2009年06期 【DOI】CNKI:SUN:ZSYJ.0.2009-06-017