陈自宏; 虞泓; 曾文波; 杨俊媛; 杨钟林; 袁静
云南大学中草药生物资源研究所云百草实验室
【中文摘要】 为了提高蛹草拟青霉cmily-02产虫草素得率,采用正交实验优化固体发酵培养基配方。以虫草素产量为指标,通过高效液相色谱法检测,筛选出最优固体培养基为:大米粉2.0%、青稞粉0.5%、玉米粉3.0%、黄豆粉0.5%、粗麦麸30%、KH2PO3 0.05%、MgSO4 0.1%。从培养温度和干燥温度2方面,探讨了温度对虫草素产量的影响,得出产虫草素的最佳培养温度为28℃,最佳干燥温度为30℃。通过固体发酵条件优化,虫草素产量可达到1 850μg.g-1。
【英文摘要】 In order to increase the yield of cordycepin in the culture mycelia of Paecilomyces militaris cmily-02,the best solid culture medium was screened by orthogonal tests and the content of cordycepin in the culture mycelia was determined by HPLC.The results showed that the optimum medium components were rice flour 2.0%,highland barley flour 0.5%,indian meal 3.0%,soybeans meal 0.5%,outerbran 30%,KH2PO4 0.05%,MgSO4.7H2O 0.1% and so on.The optimum culture conditions were culture temperature 28℃,drying temperature 30℃ with the total yield of cordycepin reaching as high as 1 850 μg.g-1.
【中文关键词】 蛹草拟青霉; 固体发酵; 正交实验; HPLC
【英文关键词】 Paecilomyces militaris; Solid fermentation; Orthogonal test; HPLC
【基金】云南省自然科学基金重点项目“云南虫草遗传资源及其开发利用研究”(2008CC019)
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2009年06期 【DOI】CNKI:SUN:ZSYJ.0.2009-06-020