李翠翠; 尉玉晓; 郭立忠
青岛农业大学生命科学学院农业应用真菌研究室
【中文摘要】 实验研究了桑黄液体发酵培养基中不同营养组成对桑黄菌丝生长的影响。以菌丝生物量和多糖产量为主要指标,对桑黄液体发酵培养基进行了优化。通过单因素试验和正交试验筛选出了桑黄液体发酵的最佳培养基。单因素试验结果表明最适氮源为麦麸,最佳碳氮比为20:1;正交试验结果表明,优化培养基配方为玉米粉50g·L-1、麦麸50g·L-1、KH2PO42g·L-1、MgSO41g·L-1、VB1300μg·L-1、VB2400μg·L-1。
【英文摘要】 The effects of cultural compounds on the growth of Phellinus igniarius mycelium were investigated.Taking the mycelial biomass and production of polysaccharide as detecting indices,the optimum submerged fermentation parameters for Phellinus igniarius were studied by single factor test and orthogonal test.The result by single factor test showed that the optimum nitrogen source was bran and C/N was 20:1.Through orthogonal test,the optimum formula for submerged fermentation were proposed of maize powder 50 g·L-1,bran 50 g·L-1,KH2PO4 2 g·L-1,MgSO4 1 g·L-1,VB1 300 μg·L-1,VB2 400 μg·L-1.
【中文关键词】 桑黄; 液体发酵; 生物量; 多糖
【英文关键词】 Phellinus igniarius; Liquid fermentation; Biomass; Polysaccharide
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2009年02期 【DOI】CNKI:SUN:ZSYJ.0.2009-02-017