关雪娟; 许璐; 邵彦春; 王卓仁; 陈福生; 罗信昌
湖北华中农业大学食品科技学院; 湖北华中农业大学食品科技学院; 湖北华中农业大学食品科技学院; 湖北华中农业大学植物科技学院; 湖北华中农业大学食品科技学院; 湖北华中农业大学植物科技学院 武汉430070; 武汉430070; 武汉430070; 武汉430070; 武汉430070; 武汉430070
【中文摘要】 利用简单重复序列间扩增多态性(ISSR)对12株蘑菇属菌株(其中菌株S1、S2为河北康保分离的菌株,具自主知识产权)和3株属外参照菌株进行了遗传多样性分析,构建了遗传相关聚类图。本研究结果表明,河北康保分离的两株菌株S1和S2与现存生产上应用的同类菌种存在明显差异,同时也表明ISSR分子标记在蘑菇品种的鉴别和菌株亲缘关系分析上具有较好的适用性。
【英文摘要】 12 Agaricus strains (including 2 strains which were isolated from Kangbao in Hebei province with self-owned intellectual property rights) and 3 out-genus reference strains were analyzed for the genetic diversity using inter simple sequence repeats (ISSR). The results in this paper revealed that these two isolated strains S1 and S2 had great differences with those strains in the similar species which were used in production nowadays. ISSR was a suitable molecular marker for species differentiation and genetic analysis among close related strains of Agaricus strains.
【中文关键词】 蘑菇菌株; 多态性; 聚类分析
【英文关键词】 Agaricus strains; Polymorphism; Cluster analysis
【基金】中国食用菌协会资助项目
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2007年03期 【DOI】CNKI:SUN:ZSYJ.0.2007-03-017