王晓娥; 姚方杰
吉林农业大学园艺学院; 吉林农业大学园艺学院 吉林长春130118; 吉林工程技术师范学院生物与食品工程系; 吉林长春130052; 吉林长春130118
【中文摘要】 以我国云南和四川产的5个块菌菌株为试验材料,提取块菌DNA并对rDNA的ITS序列分析试验条件的初步研究,结果表明:运用常规CTAB法提取块菌子实体DNA,获得的DNA产量高,可以满足本试验的要求;明确了rDNA ITS区域的适宜PCR扩增和克隆条件;获得4个长度为650bp、1个长度为800bp的PCR产物;将PCR产物连接到PMD18-T载体上,转化E.coliJM109,获得了阳性克隆,供进一步序列分析用。
【英文摘要】 5 truffle samples clustered from Sichuan and Yunnan were selected to extract DNA and the internal transcribed spacer(ITS) regions of nuclear ribosomal DNA was amplified.The results showed: The optimal PCR and clone conditions were assured;The length of 4 samples was 650bp and1 sample was 800bp;The PCR product was linked to PMD18-T vector and transformed into E.coliJM109.We chose the positive clone which would use in the further sequence analysis.
【中文关键词】 块菌; rDNA; PCR; 克隆
【英文关键词】 Truffle; rDNA; PCR; Clone
【基金】农业部“98”资助项目(项目编号2001-21)
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2006年05期 【DOI】CNKI:SUN:ZSYJ.0.2006-05-015