王伟; 尚德静; 温磊
辽宁师范大学生命科学学院; 辽宁师范大学生命科学学院; 辽宁师范大学生命科学学院 大连116029; 大连116029; 大连116029
【中文摘要】 建立了灵芝发酵菌丝三萜类化合物含量的检测方法。方法以齐墩果酸作为标准品,用香草醛-高氯酸发色体系进行比色,确定灵芝发酵菌丝三萜含量测定的实验条件。结果三萜类在20-140ug范围内有很好的线性关系,y=0.005x-0.019 r=0.998结论本法简便、准确、重复性好,可用于灵芝发酵菌丝三萜类含量的检测,以鉴定灵芝的品质及其产品的三萜含量。
【英文摘要】 objective: To establish a method of quantitative analysis of triterpernoid in mycelia of Ganoderma lucidum.Method : the content of triterpenoid in mycelia in Ganoderma lucidum was estimated by using the colorimetric method of vanillin perchloric acid under the conditions of oleanolic acid as a standard.Result: its standard curve was A=0.005C-0.019 with a relativity coefficient of 0.998 and the liner range was between 20140ug.Conclusion: this method is proved to have the advantages of easy operation,high accuracy and well repeatability and it can be used for the quantitative analysis of triterpenoid in mycelia of Ganoderma lucidum.
【中文关键词】 灵芝; 菌丝; 三萜化合物; 含量测定
【英文关键词】 Ganoderma lucidum; mycelium; triterpenoid; content determination
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2006年01期 【DOI】CNKI:SUN:ZSYJ.0.2006-01-013