孙培龙; 赵明珠
浙江工业大学轻工系; 浙江工业大学轻工系 杭州 310014; 杭州 310014
【中文摘要】 本文报道了一种以纸电泳分析香菇嘌呤的方法,该法能很好地分离各种核苷酸干扰物,回收率为89％,相对误差5％。本文重点报导了从香菇柄中提取香菇嘌呤的方法,研究证实菇柄按1:8～10的固液比加75％(v/v)的乙醇在自然状况下浸泡6hr,捣至2mm×5mm的浆状,于83～85℃水浴中回流萃取2hr,香菇漂呤的提取得率可达161mg％,比资料报道的70mg％高2.3倍。
【英文摘要】 A analytical method for eritadenine using paper electrophoresis was reported. The interfer- ences of the vary nucleotides could be seperated. The rate of recovery of this method was 89%, and the rela- tive error was 5%. A extracting method of eritadenine from the stem of Lentinus edodes was also reported. The better technique of extraction was proofed as follows : A portion of the stem of L. edodes was naturally soaked with 810 portion of 75% (v/v) alcohol for 6 hours, then was pounded to small pieces which the size was 2mm× 5mm. After that, the pulp was extracted for 2 hours at 8385℃. The rate of extraction of eritadinine was 161 mg%, it was 2.3 times as much as 70mg% that was reported by the reference.
【中文关键词】 香菇嘌呤; 提取; 分析测定
【英文关键词】 eritadenine; extraction; analysis; determination
【基金】浙江省科委项目
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,1997年02期 【DOI】CNKI:SUN:ZSYJ.0.1997-02-015