程东升; 邹莉; 潘学仁
东北林业大学
【中文摘要】 探讨了外生菌根菌耳环乳牛肝（Ｓｕｉｌｌｕｓｇｒｅｖｉｌｌｅｉ）的原生质体制备及再生条件。用ＭＹＧ培养基培养菌丝７天、宋用１．５％Ｌｙｗａｌｌｚｙｍｅ，以０．６ｍｏｌ甘露醇为稳渗剂，在酶解液ｐＮ值５．５，温度３１℃，酶解４ｈｒ的条件下，每０．１ｇ湿菌丝的原生质体产量这６×１０￣６／ｍｌ。用ＳＯ培养基培养菌丝能提高原生质体产量，但再生率下降。综合产量和再生率考虑以ＭＹＧ培养菌丝效果较好。
【英文摘要】 Conditions for preparingthe protoplasts from mycelia of ectomycorrhizal fungus SMillus grevillei were examined.The highest production of protoplasts(6 ×10￣6/ml per 0.1g fresh mycelia) was abtained with the enzyme mixture of pH 5.5 containing 1.5% Lywallzyme,0.6mnol/L mannitol as osmotic stabilizer,and incubated at 31℃, from 7-day age mycelia cultured in MYG medium. UsingSO miedium instead of MYG doubled the protoplast productlon, but markedly decreased the plating efficiency. It is concl-uded that MYG is a better medium than SO for the preparation of protoplasts from Suillus previlllei.
【中文关键词】 外生菌根菌; 原生质体制备; 细胞工程
【英文关键词】 Ectomycorrhizal fungi; Protopiast prepartion; Cytological engi-neering
【基金】国家教委资助基金
【文献出处】 中国食用菌,EDIBLE FUNGI OF CHINA,编辑部邮箱,1994年02期 【DOI】CNKI:SUN:ZSYJ.0.1994-02-009