曾繁华; 魏征宇; 罗文; 薛喜文
江西大学生物系; 江西大学生物系; 江西大学生物系; 江西大学生物系 南昌330047; 南昌330047; 南昌330047; 南昌330047
【中文摘要】 柠檬酸工业废液中含有一定有效物质,如糖类、蛋白质、矿物质等,可供微生物利用。本文介绍了利用废液深层培养双孢蘑菇菌丝蛋白的可能性及条件。并用1吨发酵堆进行生产试验,探讨了生产工艺条件,分析了发酵液中底物、产物的动态变化。双孢蘑菇菌丝蛋白培养温度25℃,底物 pH6,发酵周期6天,菌球直径1～3mm,菌球数量4.8×103个/ml,菌丝温重达底物量的29.3%。在上述条件下,发酵液中菌球直经、菌丝数量、发酵液粘度受生物剪切作用的影响,粘度大,剪切作用大,菌球直径小,菌球数量则多。本文为新型单细胞蛋白的开发,促进物质的多层次利用,消除环境污染,提供了一个新途径。
【英文摘要】 The waste from-citric acid production consists of some useful materials,such as sugar, proteins and minerals,which can be utilized by microorganisms.What is described in this paper is the possability and conditions of using waste from citric acid production in a deep culture of the mycelial proteins of Agaricus bisporus.The test was made in a one-ton for- menter.The technological conditions were studied and the kinetic changes of the substrates and the products in ferment fluid were analyzed.The conditions in which the mycelical proteins of Agaricus bisporus were cultued in a large fermenter are:temperature,250;pH of the substrate,6.0;and the period of the fermentation,6 days.The diameter of the thallus can reach to 1—3mm and the wet weight of it to 29.3%.The results show a new way in exploition new-type single-cell protein,in making good use of ferment materials, and ha protecting the environment from pollution.
【中文关键词】 单细胞蛋白; 双孢蘑菇
【英文关键词】 Single-cell protein Agaricus bisporus
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,1993年01期 【DOI】CNKI:SUN:ZSYJ.0.1993-01-002