突脐孢属Brnl基因核苷酸序列比较及系统发育研究
SEQUENCE COMPARISON AND PHYLOGENETIC ANALYSIS OF BRN1 GENES FROM EXSEROHIL UM SPECIES
孙广宇 张雅梅 张荣
摘 要：对所有供试突脐孢菌株的Brnl基因(1,3,8-三羟基萘还原酶基因)扩增均获得PCR产物.序列比较表明:在种内各菌株间没有核苷酸序列长度变化,存在核苷酸序列简单代换;在种间核苷酸序列长度有变化,核苷酸的缺失或插入发生在内含子区;所有菌株编码区核苷酸序列长度相同;在种内水平氨基酸序列没有差别,显示出高度的保守性.利用最大简约法(Maximum Parsimony)和邻近结合法(Neighbor-joining)构建系统发育树,两个系统发育树的拓扑结构相似,不同种在不同的分支上.Brn1基因适合突脐孢属种级水平的分子系统学研究.
关键词：系统发育树;离蠕孢属;序列分析
分类号：Q939.97 文献标识码：A
文章编号：1672-6472(2004)04-0480-0486
基金项目：国家自然科学基金资助项目(项目编号:30170004)
作者简介：孙广宇,通讯作者 E-mail:sunguang@public.xa.sn.cn
作者单位：孙广宇（西北农林科技大学植保学院,陕西,杨凌,712100）
张雅梅（西北农林科技大学植保学院,陕西,杨凌,712100）
张荣（西北农林科技大学植保学院,陕西,杨凌,712100）
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收稿日期：2004年3月8日
修稿日期：2004年5月20日
出版日期：2004年11月22日
菌物学报
MYCOSYSTEMA
2004 Vol.23 No.4 P.480-486
